ReagenX
Urinary Tract Infection (UTI) & DR Panel (Fluorescence PCR method)
- Urinary Tract Infection (UTI) & Drug Resistance (DR) Gene Detection Kit is designed with specific primers and probes for different common pathogens and drug resistance genes. Polymerase chain reaction (qPCR) and multiple fluorescent probe technique are used to amplify and detect specific nucleic acid sequences of pathogens and drug resistance genes listed in Table 1.
- By adding edible yeast as internal control (IC), the extraction and detection process of pathogen nucleic acid in urine is monitored to effectively avoid false negative results. In order to avoid aerosol contamination of the amplified products, the UDG enzyme /dUTP system was added to the amplification system to effectively degrade the amplified products and avoid false positive results.
- This kit is a fully premix freeze-dried system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes. A total of 1-8 Wells are lyophilized powders for different target genes. Detection can be performed directly after adding dissolving solution and extracted nucleic acid.
Model: PR2026-UT01
Format: Test cassette
Storage: -25~-15℃(-13~-5℉)
Shelf life: 12 months
| Components | 8 samples/kit | 24 samples/kit | Ingredient |
|---|---|---|---|
| UTI Lyophilized Reagent | 8×8 strip tubes | 24×8 strip tubes | Specific primer&probes for the detection of pathogens and DR genes in Table 1, dNTP/dUTP Mix, Mg2+, Taq polymerase and UDG enzyme |
| UTI Lysis Buffer | 1 mL×1 tube | 1 mL×3 tubes | Surface active agent, balanced salt solution |
| Internal Control Dry Powder | 1 tube | 1 tube | Edible yeast powder |
| Internal Control Solution | 1 mL×1 tube | 1 mL×1 tube | Dnase/Rnase-free H2O |
| One Test Glass Beads | 8 tubes | 8 tubes×3 bags | Glass beads |
| UTI Positive Control | 300 μL×1 tube | 300 μL×1 tube | Plasmid containing every target gene sequence |
| UTI Negative Control | 300 μL×1 tube | 300 μL×1 tube | Plasmid containing internal control sequence |
| UTI Dissolving Solution | 1 mL×1 tube | 1 mL×3 tubes | Stabilizer |
NOTE:
1. The kit can be transported at room temperature. (No more than 1 month)
2. The reagents repeated freezing and thawing should not exceed 7 times.
1. Multiple quantitative analysis
19 common pathogen nucleic acids and 12 common drug resistance genes
2. Rapid response
With a fast PCR instrument, the test can be completed in 30 minutes
3. Application scenarios
Urology routine testing/third-party testing laboratory/inspection department
Postoperative monitoring/disease control/scientific research units/doctors cooperate to publish articles
4. Product advantages
Freeze-dried system
Reduce transportation risks and increase stability
No extraction equipment required
1. Disposable powder free gloves & other personal protective equipments.
2. Pipettes (adjustable) & Sterile pipette tips.
3. Urine sample collection device.
4. 1.5 mL centrifuge tubes and racks.
5. Bench top centrifuge for centrifuge tubes and PCR tubes.
6. Vortex mixer(Power ≥40W).
7. Centrifuge tube holder for vortex mixer.
8. Metal bath/water bath (1.5mL centrifuge tube, 95℃).
9. Real-time PCR instrument with FAM/VIC/ROX/CY5 fluorescence channels (ABI7500, Bio-rad CFX96, QuantStudio, SLAN-96S, BTK-96).
1). Detection limitation: 200 CFU/mL
2). Precision: The coefficient of variaation (CV, %) of Ct value of within-batch/between-batch precision is ≤5%
3). Accuracy: The conformity rate of negative/positive reference: 100%
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Urinary Tract Infection (UTI) & DR Panel (Fluorescence PCR method)
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