The kit is designed with specific primers and probes for different common pathogens and drug resistance genes. Polymerase chain reaction (qPCR) and multiple fluorescent probe technique are used to amplify and detect specific nucleic acid sequences of pathogens and drug resistance genes listed in Table 1. 

By adding edible yeast as internal control (IC), the extraction and detection process of pathogen nucleic acid from wound swab is monitored to effectively avoid false negative results. In order to avoid aerosol contamination of the amplified products, the UDG enzyme /dUTP system was added to the amplification system to effectively degrade the amplified products and avoid false positive results. 

This kit is a fully premix freeze-dried system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes. A total of 1-8 Wells are lyophilized powders for different target genes. Detection can be performed directly after adding dissolving solution and extracted nucleic acid.